Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the potent antibiotic taromycin

1. Experimental methods 2. Strains and plasmids used in this study (Table S1) 3. Deduced functions and sequence comparison of the tar genes (Table S2) 4. Schematic diagram of the tar gene cluster direct cloning (Figure S1) 5. Restriction mapping of pCAP01-tar and its derivatives (Figure S2). 6. Results of heterologous expression of the tar gene cluster in S. coelicolor M1146 (Figure S3) 7. Results of genetic complementation of the tar regulatory genes (Figure S4–S7) 8. NMR, MS and Marfey analysis tables (Table S3–S5, Figure S8–S11) 9. Bioactivity of taromycin A compared to daptomycin (Table S6) 10. Verified functionality of pCAP01 by direct cloning and expression of the marinopyrrole biosynthesis (mpy) gene cluster (Figure S12) 11. Reference list for supporting information